Submit an abstract

Guidelines for submission

Submissions for the 2015 event are presently NOW OPEN

Please read below for submission details

Submissions deadline is the 7th August 2015 

Submission process: Please send submissions with your preference of talk (oral or poster) to

cintia.dias@uon.edu.au

 Please follow the following format in submitting your abstract

– Submissions can be for oral or poster presentations (please indicate preference)

– Submit a single paragraph abstract of <250 words prepared in English (see example below)

– Please use 12 point Times New Roman with title in bold and authors on separate line in italics

Please quote any references (2 maximum) in the text in this format: (Name, Initial of first author only, Abbreviated journal title (Year;Volume:first page) – see below for example.

 

Guidelines for submission 2015 (Click on title to download word version)

In-vitro Δ6-desaturase activity: Fatty acid and lipid modification of isolated chloroplasts from borage leaves and microalgae Isochrysis galbana.

Kathrin Stähler, Siew-Young Quek, Matthew R. Miller

This study investigated the ability of isolated chloroplasts from borage (Borago officinalis L.) and the microalgae Isochrysis galbana to modify lipids in-vitro in order to assess Δ6-desaturase activity. Chloroplasts from borage leaves and Isochrysis galbana were incubated alone (control) or added with one of the following precursors (30µg precursor/25µg chlorophyll): 1) high oleic acid (C18:1n-9) free fatty acids (OA-FFA), 2) high α-linolenic acid (C18:3n-3) FFA (ALA-FFA) or 3) high ALA- monogalactosyl diacylglycerol (ALA-MGDG). Sampling took place every 30 min during the trial period and oil was extracted. Changes in fatty acid (FA) composition were determined by GC-MS and those in lipid class by Iatroscan thin layer chromatography-FID. We demonstrated that Δ6-desaturase activity (measured by changes in SDA/ALA ratio) was enhanced by up to 53% during incubation, when adding ALA-FFA to the microalgae chloroplasts. However, the addition of OA-FFA to the isolated chloroplasts of both species showed no significant (P < 0.05) changes in major FA during in-vitro incubation. For borage samples, results indicated no incorporation of the added FFA to chloroplast lipids. For Isochrysis galbana samples, there is evidence showing the incorporation of the added FFA precursors into chloroplast glycerolipids with FFA in the ALA-FFA and OA-FFA samples decreasing from 8.4% to 2.6% and 8.4% to 2.2%, respectively after 30 min of incubation. Current results indicate that chloroplasts of Isochrysis galbana have the better ability to incorporate the added FFA precursors (OA-FFA and ALA-FFA) into chloroplast lipids and show more promising Δ6-desaturate activity compared with borage chloroplasts (Stähler K JOACS 2011;88:1715).

 

Affiliations

Kathrin Stähler. The University of Auckland;

Siew-Young Quek. The University of Auckland;

Matthew R. Miller. New Zealand Institute for Plant & Food Research;

 

Abstracts deviating from this format will be returned to author for re-writing.

 

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